Number of allele specific oligonucleotide primers and dual labeled probes that Most innovative features in the program, helps design the fewest Real time PCR primer & probe efficiency is enhanced by avoiding template secondary structures. Highly specific oligos are designed by avoiding regions of significant homologies found by automatically interpreting BLAST search results. Sophisticated Algorithms for Assay Success This powerful functionality is sure be helpful for many challenging tasks such as detection, identification, quantification or monitoring of contaminants, environments. For related organisms, AlleleID® can be used to study gene expression when genome draft of the organism under study is not available. Strain (strain detection) or species of interest from the mix.įor cross species assays, AlleleID® identifies the conserved regions to design a universal probe. These assays are designed to detect only the Time PCR primer design ( SYBR® Green primer design included) and dual labeled probe design ( TaqMan® probes, TaqMan® MGB probes and molecular beacons). Identification Assays/Cross Species Assays/AlleleĪnalyzes conserved and species specific regions. AlleleID® also offers support for designing microarray experiments for detecting alternative splicing events. With ClustalW multiple sequenceĬan be used to design species identification/cross species probes for microarrays or real time PCR including SYBR® Green, TaqMan® MGB, TaqMan® probes, Molecular Beacons AlleleID® Assay Design for Bacterial Identification and More.ĭesktop tool designed to address the challenges of bacterialĭetection or species identification.

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